Determination of biochemical indicators is a number of specific analytic tests that can be determined in the pooled sample serum of animals or in the serum of some individuals. In our study we have movement biochemical parameters in the blood serum of cows at different stages. The study was conducted on 12 cows in the dry period, which were followed on, freshly calved cows and the cows in early lactation, and 12 randomly selected cows in the high stage of lactation. Blood samples were taken by puncture of the tail vein (vena caudalis) and then centrifuged at 3000 rpm/10 min. In the obtained serum there were determined following parameters: glucose, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamyl transferase, urea, total protein, BEFA, beta-hydroxybutyric acid, albumin, iron, cholesterol, HDL, LDL, triglycerides, calcium and phosphorus. Except of biochemical parameters, respiratory rate, temperature and score of BSC were measured. The conducted analysis revealed that the cows in the dry period had a satisfactory stage of energy, protein and mineral balance, while cows in freshly calved stage, some cows, had unsatisfactory state of energy balance, proteins and minerals. In early lactation stage trend of negative energy balance continues, concentration of albumin and protein is low, and mineral status is unsatisfying, while the cows in the top of lactation have better energy and protein supply than at the beginning of lactation. High concentration of urea and phosphorus deficency indicates that the protein is in excess in regards of energy, so we should take a serious step in correcting a diet.