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Influence of different light spectrum and hormone concentration on in vitro growth of garlic explants
Sunčica Guberac (2017)
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechical Sciences Osijek
DEPARTMENT FOR PLANT PRODUCTION
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Guberac, S. (2017). Utjecaj različitog spektra svjetla te koncentracije hormona na rast eksplantata češnjaka in vitro (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:261562

Guberac, Sunčica. "Utjecaj različitog spektra svjetla te koncentracije hormona na rast eksplantata češnjaka in vitro." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:151:261562

Guberac, Sunčica. "Utjecaj različitog spektra svjetla te koncentracije hormona na rast eksplantata češnjaka in vitro." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:151:261562

Guberac, S. (2017). 'Utjecaj različitog spektra svjetla te koncentracije hormona na rast eksplantata češnjaka in vitro', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek, accessed 25 June 2019, https://urn.nsk.hr/urn:nbn:hr:151:261562

Guberac S. Utjecaj različitog spektra svjetla te koncentracije hormona na rast eksplantata češnjaka in vitro [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek; 2017 [cited 2019 June 25] Available at: https://urn.nsk.hr/urn:nbn:hr:151:261562

S. Guberac, "Utjecaj različitog spektra svjetla te koncentracije hormona na rast eksplantata češnjaka in vitro", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek, Osijek, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:151:261562

Metadata
TitleUtjecaj različitog spektra svjetla te koncentracije hormona na rast eksplantata češnjaka in vitro
Title (english)Influence of different light spectrum and hormone concentration on in vitro growth of garlic explants
AuthorSunčica Guberac
MentorNada Parađiković (mentor)
Committee memberJasenka Ćosić (predsjednik povjerenstva)
Committee memberTomislav Vinković (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechical Sciences Osijek
(DEPARTMENT FOR PLANT PRODUCTION)
Osijek
Defense date and country2017-05-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Genetics and Breeding of Plants, Animals and Microorganisms
Abstract
Primjena tehnika kulture tkiva i stanica u proizvodnji češnjaka započela je 70-ih godina prošloga stoljeća. Navedene tehnike pokazale su se boljima u odnosu na klasičnu reprodukciju češnjevima, budući da zahtijevaju samo stanice ili male dijelove tkiva za dobivanje velikog broja biljaka. Kvaliteta svjetlosti predstavlja jedan od najvažnijih okolišnih čimbenika čijom kontrolom je moguće regulirati in vitro rast i razvoj. Odabirom koncentracije i vrste hormona, kao ključne komponentne hranjive podloge, moguće je odrediti smjer razvoja biljnih stanica u kulturi. Cilj ovoga istraživanja bio je utvrditi utjecaj različitog spektra svjetla i koncentracije hormona na početni porast eksplantata češnjaka in vitro. U istraživanje su bila uključena dva ekotipa češnjaka, Slavonski ozimi (Hrvatska) i Vincek (Slovenija). Kao eksplantati korišteni su dijelovi klice češnjeva češnjaka. Hranjiva podloga pripremljena je prema recepturi Linsmaier i Skoog (1965.). Za istraživanje su korištene dvije različite varijante hranjive podloge (1,0 mg/L BAP i 1,5 mg/L BAP) te dvije varijante osvjetljenja (bijelo svjetlo i plavo svjetlo). Nakon dva tjedna uzgoja u kulturi određeni su masa, duljina i multiplikacijski indeks ispitivanih ekotipova. Utvrđen je statistički značajan utjecaj (p < 0,01) koncentracije hormona i interakcije svjetlost x hormon na sve ispitivane parametre kod oba ekotipa češnjaka. Najveća duljina i masa češnjaka Vincek, kao i najveći postotak preživljavanja, zabilježeni su kod tretmana plavim svjetlom uz 1,5 mg/L BAP. Najveća duljina i masa Slavonskog ozimog češnjaka, kao i najveći postotak preživljavanja, zabilježeni su kod tretmana bijelim svjetlom uz 1,5 mg/L BAP.
Abstract (english)
The use of tissue and cell culture techniques in garlic production began in the 1970s. These techniques have been shown to be superior to classical reproduction by cloves, as they only require cells or small parts of the tissue to obtain a large number of plants. Light quality is one of the most important environmental factors which can regulate in vitro growth and development. Hormones represent a key medium component and by selecting their concentration and type it is possible to define the direction of plant cell growth in culture. The aim of this study was to determine the influence of different light spectrum and hormone concentration on the initial growth of garlic in vitro. The study included two garlic ecotypes, Slavonian winter (Croatia) and Vincek (Slovenia). Clove germs were used as explants. Culture medium was prepared according to Linsmaier and Skoog (1965). Two different variants of hormon concentration (1.0 mg/L BAP and 1.5 mg/L BAP) and light spectrum (white light and blue light) were used as treatments. After two weeks of cultivation, growth parameters (mass, length and multiplication index) were measured. Results showed a statistically significant effect (p < 0.01) of the hormone concentration and the interaction of light x hormone on all examined parameters (mass, length and multiplication index) in both garlic ecotypes. The highest length and mass of garlic Vincek, as well as the highest survival rate, were observed at blue light + 1.5 mg/L BAP treatment. The highest length and mass of Slavonian winter garlic and the highest survival rate, were observed at white light + 1.5 mg/L BAP treatment.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:261562
Study programmeTitle: Povrćarstvo i cvjećarstvo
Study programme type: sveučilišni
Study level: diplomski
Academic / professional title: magistar/ magistra inženjer/inženjerka povrćarstva i cvjećarstva (magistar/ magistra inženjer/inženjerka povrćarstva i cvjećarstva)
Type of resourcetext
File originBorn digital
Access conditionsOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
Public notediplomski rad
Created on2017-06-28 08:24:20