Appearance of Phylloxera made grape vine growth impossible without grafting susceptible vine varieties to resistant rootstocks. Proper rootstock controls Phylloxera and enables grape vine breeding. In continental parts of Croatia grafting is performed with Phylloxera-resistant American rootstocks which possess resistance through their increased cell layer thickness as compared to susceptible European vines. Ones of the most frequently used are 5BB and SO4 rootstocks. In this research we aimed to analyze the influence of Kober 5BB, Kober 125AA and SO4 rootstocks on to sugar levels, level of acids and pH of riesling, graševina, sauvignon blanc, chardonnay and traminer grape must. Analyses took part in Mandićevac, experimental field of Faculty of Agriculture, University of Osijek within Đakovo vineyards which belong to eastern continental part of Croatia vineyard region, Slavonia sub region. The research was performed during the year 2015. Grape must Riesling contained highest sugar levels onto Kober 5BB rootstock (95 ⁰Oe), while higher acid level was detected onto SO4 rootstock (8,00 g/l). Highest pH values were measured onto Kober 5BB rootstock (3,21). While graševina grape must revealed highest levels of sugar (100 ⁰Oe), highest acidity (6,0 g/l) and pH values (3,42) onto SO4 rootstock, chardonnay grape must revealed highest sugar levels (105 ⁰Oe), acid levels (6,80 g/l) and pH values (3,50) onto Kober 5BB rootstock. sauvignon blanc grape must sugar levels were increased on to Kober 5BB rootstock (107 ⁰Oe), as well as total acid levels (8,20 g/l), as compared to rootstock SO4, which grafting resulted in higher pH value (3,41) of this grape must. Finally, traminer grape must had highest levels of sugar on to 125AA rootstock (100 ⁰Oe) most prominent acid values on to Kober 5BB rootstock (4,9 g/l) and highest pH values on to SO4 rootstock (3,83).