There is a lot of evidence that indicates that mycorrhiza is the best and most effective method for growing plants, their growth and development, and obtaining higher yields and healthier food. Many producers consider it the right solution for growing different crops and protecting the ecosystem. It is applicable in numerous production areas and promotes the cultivation of crops on soils with difficult growing conditions. By applying mycorrhiza, considerable savings are made on the consumption of water, fertilizers and pesticides. Over 90 % of plant species create a symbiotic root relationship with mycorrhizal fungi naturally. In this thesis, an experiment was set up in containers to determine the influence of mycorrhizal fungi and different lighting on the growth of young industrial hemp plants. According to the results of thethesis, the mass of the roots of young hemp plants was on average 0.08 g, the mass of the stem 0.39 g, the mass of the plant itself 0.48 g, and the mass of the leaves of one plant 0.18 g. From the morphological characteristics of young hemp plants, the length of the root was on average 5.3 cm, the length of the stem 15.5 cm and the length of the whole plant 21.0 cm. Lastly, there were an average of 5 leaves per hemp plant. Treatments under white light prevailed for the most part in terms of greater plant mass and length, only blue light had a significant effect on the number of leaves per plant (6 leaves) compared to white light (4 leaves) and on some other parameters (root mass at blue light is on average 0.01 g higher, stem mass is 0.05 g higher in blue light, and leaf mass is 0.02 g higher in blue light), but the differences are almost imperceptible since it is a very small deviation.