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Scientific paper - Original scientific paper
COMPARISON OF COMMERCIAL DNA KITS AND TRADITIONAL DNA EXTRACTION PROCEDURE IN PCR DETECTION OF PORK IN DRY/FERMENTED SAUSAGES
Poljoprivreda, 21 (2015), 1 SUPPLEMENT; 199-202. https://doi.org/10.18047/poljo.21.1.sup.47
Djurkin Kušec, Ivona; Radišić, Žarko; Komlenić, Miodrag; Kušec, Goran

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Djurkin Kušec, I., Radišić, Ž., Komlenić, M. & Kušec, G. (2015). COMPARISON OF COMMERCIAL DNA KITS AND TRADITIONAL DNA EXTRACTION PROCEDURE IN PCR DETECTION OF PORK IN DRY/FERMENTED SAUSAGES. Poljoprivreda, 21. (1 SUPPLEMENT), 199-202. doi: 10.18047/poljo.21.1.sup.47

Djurkin Kušec, Ivona, et al. "COMPARISON OF COMMERCIAL DNA KITS AND TRADITIONAL DNA EXTRACTION PROCEDURE IN PCR DETECTION OF PORK IN DRY/FERMENTED SAUSAGES." Poljoprivreda, vol. 21, no. 1 SUPPLEMENT, 2015, pp. 199-202. https://doi.org/10.18047/poljo.21.1.sup.47

Djurkin Kušec, Ivona, Žarko Radišić, Miodrag Komlenić and Goran Kušec. "COMPARISON OF COMMERCIAL DNA KITS AND TRADITIONAL DNA EXTRACTION PROCEDURE IN PCR DETECTION OF PORK IN DRY/FERMENTED SAUSAGES." Poljoprivreda 21, no. 1 SUPPLEMENT (2015): 199-202. https://doi.org/10.18047/poljo.21.1.sup.47

Djurkin Kušec, I., et al. (2015) 'COMPARISON OF COMMERCIAL DNA KITS AND TRADITIONAL DNA EXTRACTION PROCEDURE IN PCR DETECTION OF PORK IN DRY/FERMENTED SAUSAGES', Poljoprivreda, 21(1 SUPPLEMENT), pp. 199-202. doi: 10.18047/poljo.21.1.sup.47

Djurkin Kušec I, Radišić Ž, Komlenić M, Kušec G. COMPARISON OF COMMERCIAL DNA KITS AND TRADITIONAL DNA EXTRACTION PROCEDURE IN PCR DETECTION OF PORK IN DRY/FERMENTED SAUSAGES. Poljoprivreda [Internet]. 2015 September 02 [cited 2024 December 19];21(1 SUPPLEMENT):199-202. doi: 10.18047/poljo.21.1.sup.47

I. Djurkin Kušec, Ž. Radišić, M. Komlenić and G. Kušec, "COMPARISON OF COMMERCIAL DNA KITS AND TRADITIONAL DNA EXTRACTION PROCEDURE IN PCR DETECTION OF PORK IN DRY/FERMENTED SAUSAGES", Poljoprivreda, vol. 21, no. 1 SUPPLEMENT, pp. 199-202, September 2015. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:151:566565. [Accessed: 19 December 2024]

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Metadata
Title (english)COMPARISON OF COMMERCIAL DNA KITS AND TRADITIONAL DNA EXTRACTION PROCEDURE IN PCR DETECTION OF PORK IN DRY/FERMENTED SAUSAGES
AuthorIvona Djurkin Kušec
AuthorŽarko Radišić
AuthorMiodrag Komlenić
AuthorGoran Kušec
Author's institutionJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Animal Production and Biotechnology)
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Production and Processing of Animal Products
Abstract (english)
In the present study four commercially available DNA extraction kits (Wizard® Genomic DNA Purification Kit, High Pure PCR Template Kit, DNeasy mericon Food and GeneJET PCR Purification Kit), as well as standard phenol/chloroform isolation technique have been evaluated regarding their concentration, purity and suitability for amplification of porcine DNA in dry/fermented sausages. The isolates were assessed for quantity and quality using spectrophotometer (IMPLEN GmbH, Germany). To verify template usability and quality of isolated DNA, the polymerase chain reaction (PCR) targeting at porcine cytochrome b by species specific primers was used. The comparison of extraction methods revealed satisfactory efficiency and purity of all extraction kits, while with standard phenol/chloroform isolation method high concentrations of DNA with low A260/280 were obtained. However, all the investigated techniques proved to be suitable for identification of porcine DNA in dry/fermented sausage. Thus, the standard phenol/chloroform DNA extraction method, as the cost-effective one, can be recommended as a good alternative to more expensive isolation kits when investigating the presence of pork DNA in dry/ fermented meat products.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - domestic
Publication versionPublished version
Journal titlePoljoprivreda
Numberingvol. 21, no. 1 SUPPLEMENT, pp. 199-202
p-ISSN1330-7142
e-ISSN1848-8080
DOIhttps://doi.org/10.18047/poljo.21.1.sup.47
URN:NBNurn:nbn:hr:151:566565
Publication2015-09-02
Document URLhttps://hrcak.srce.hr/150920
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-05-16 10:01:55