The aim of this investigation was to determine the influence of LED and FLUO lighting as well as cultivar on germination, germination energy, seedlings fresh weight and height of lettuce. Germination and germination energy were evaluated by standard laboratory procedure where seeds of three lettuce cultivars Ledenka, Nansen i Posavka were used as material. Seeds of all three cultivars were sown and at the same time placed in growth chamber equipped with LED and FLUO lamps. LED lamps were equipped with blue (440-460 nm) and red (650-670 nm) diodes. During this investigation, it was determined that lighting and cultivar significantly effects on all investigated parameters. Highest germination percentage and germination energy was determined when using LED lighting. However, significantly higher fresh weight and height of seedlings was observed in FLUO lighting. At the same time, seedlings placed under FLUO lighting had less pronounced green coloration of their cotyledons compared to that placed under LED lighting which were intensively green suggesting that they had higher chlorophyll concentration even though chlorophyll concentration was not measured during this investigation. It can be concluded that LED lighting is appropriate for testing seed germination as well as for growth and development of young lettuce plants. LED lighting is, except for increasing germination, energy efficient light source and is recommended to be installed in plant growth chambers and greenhouses because of their lightweight, small volume and long life.