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undergraduate thesis
Mikropropagacija vegetativne podloge agruma (Poncirus trifoliata Raf.) in vitro
2020. urn:nbn:hr:151:268655
Horvat, Matej
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

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Horvat, M. (2020). Mikropropagacija vegetativne podloge agruma (Poncirus trifoliata Raf.) in vitro (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:268655

Horvat, Matej. "Mikropropagacija vegetativne podloge agruma (Poncirus trifoliata Raf.) in vitro." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:151:268655

Horvat, Matej. "Mikropropagacija vegetativne podloge agruma (Poncirus trifoliata Raf.) in vitro." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:151:268655

Horvat, M. (2020). 'Mikropropagacija vegetativne podloge agruma (Poncirus trifoliata Raf.) in vitro', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 15 April 2025, https://urn.nsk.hr/urn:nbn:hr:151:268655

Horvat M. Mikropropagacija vegetativne podloge agruma (Poncirus trifoliata Raf.) in vitro [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2020 [cited 2025 April 15] Available at: https://urn.nsk.hr/urn:nbn:hr:151:268655

M. Horvat, "Mikropropagacija vegetativne podloge agruma (Poncirus trifoliata Raf.) in vitro", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:151:268655

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Metadata
TitleMikropropagacija vegetativne podloge agruma (Poncirus trifoliata Raf.) in vitro
Title (english)Micropropagation of citrus rootstock (Poncirus trifoliata Raf.) in vitro
AuthorMatej Horvat
MentorDejan Bošnjak (mentor)
Committee memberAleksandar Stanisavljević (predsjednik povjerenstva)
Committee memberMonika Tkalec (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Plant Production and Biotechnology)
Osijek
Defense date and country2020-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Fruit Growing
Abstract
U rasadničkoj proizvodnji važnu ulogu ima kontinuirano uvođenje suvremenih in vitro metoda klonskog razmnožavanja reprodukcijskog materijala. Poncirus trifoliata Raf. je najzastupljenija i najvažnija podloga mandarine Unšiu u Hrvatskoj i drugim proizvodnim zemljama svijeta. Istraživanje o mogućnosti mikropropagacije vegetativne podloge agruma Poncirus trifoliata Raf. provedeno je na Fakultetu agrobiotehničkih znanosti Osijek tijekom 2020. godine. Ispitivan je utjecaj pojedinih biljnih regulatora rasta (BAP, IBA, Zeatin i GA3) na mogućnost proliferacije i organogeneze nodijalnih eksplantata iniciranih na MS hranjivi medij. Najbolji rezultati dobiveni su na tretmanu T1 koji je uključivao kombinaciju citokinina BAP i auksina IBA. Na ovom tretmanu zabilježena je najmanja stopa kontaminacije i odumrlih eksplantata. Zdravi eksplantati T1 tretmana uključeni su u daljnju fazu multiplikacije. Nakon nekoliko uspješnih ciklusa multiplikacije dobiven je dovoljan broj novih zdravih mikrobiljaka na kojim će se obavljati brojna druga istraživanja.
Abstract (english)
The continuous introduction of modern in vitro methods in clonal propagation of plating material plays an important role in nursery plants production. Poncirus trifoliata Raf. is the most represented and most important rootstock of mandarin Unshiu in Croatia and other production countries. Research on the possibility of micropropagation citrus vegetative rootstock Poncirus trifoliata Raf. was conducted at the Faculty of Agrobiotechnical Sciences Osijek during 2020. The influence of individual plant growth regulators (BAP, IBA, Zeatin and GA3) on the possibility of proliferation and organogenesis of nodal explants initiated on MS nutrient medium was investigated. The best results was obtained on T1 treatment, which included a combination of cytokine BAP and auxin IBA. This treatment has the lowest rate of contamination and dead explants. Healthy explants of T1 treatment are included in the further phase of multiplication. After several successful multiplication cycles, a sufficient number of new healthy microplants have been successfully obtained on which numerous other researches will be performed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:268655
Study programmeTitle: Agriculture; specializations in: Agroeconomics, Plant production, Mechanization, Zootechnique, Horticulture
Course: Horticulture
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije)
Type of resourceText
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Created on2020-09-22 13:30:04