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master's thesis
Insekticidno i antifungalno djelovanje etnomopatogene gljive Beauveria bassiana i korisne bakterije Bacillus subtilis
2021. urn:nbn:hr:151:818668
Jelić, Tena
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Phytomedicine

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Jelić, T. (2021). Insekticidno i antifungalno djelovanje etnomopatogene gljive Beauveria bassiana i korisne bakterije Bacillus subtilis (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:818668

Jelić, Tena. "Insekticidno i antifungalno djelovanje etnomopatogene gljive Beauveria bassiana i korisne bakterije Bacillus subtilis." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:151:818668

Jelić, Tena. "Insekticidno i antifungalno djelovanje etnomopatogene gljive Beauveria bassiana i korisne bakterije Bacillus subtilis." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:151:818668

Jelić, T. (2021). 'Insekticidno i antifungalno djelovanje etnomopatogene gljive Beauveria bassiana i korisne bakterije Bacillus subtilis', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:151:818668

Jelić T. Insekticidno i antifungalno djelovanje etnomopatogene gljive Beauveria bassiana i korisne bakterije Bacillus subtilis [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2021 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:151:818668

T. Jelić, "Insekticidno i antifungalno djelovanje etnomopatogene gljive Beauveria bassiana i korisne bakterije Bacillus subtilis", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:151:818668

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TitleInsekticidno i antifungalno djelovanje etnomopatogene gljive Beauveria bassiana i korisne bakterije Bacillus subtilis
Title (english)Insecticidal and antifungal activity of entomopathogenic fungus Beauveria bassiana and beneficial bacterium Bacillus subtilis
AuthorTena Jelić
MentorIvana Majić (mentor)
Committee memberJasenka Ćosić (predsjednik povjerenstva)
Committee memberGabriella Kanižai Šarić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Phytomedicine)
Osijek
Defense date and country2021-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Phytomedicine
Abstract
Sintetički kemijski pesticidi već godinama predstavljaju glavni način suzbijanja štetnika i patogena. Međutm, zbog njihovog štetnog utjecaja na zdravlje ljudi i okoliš, suvremena poljoprivredna proizvodnja zahtjeva primjenu ekološki prihvatljivih metoda suzbijanja kojima pripadaju entomopatogene gljive i korisne bakterije. Cilj ovog rada je utvrditi insekticidnu i antifungalnu učinkovitost pojedinačne i kombinirane primjene entomopatogene gljive Beauveria bassiana i korisne bakterije Bacillus subtilis. Istraživanje je provedeno u laboratorijskim uvjetima, a svakih 24, 48, 72 i 96 sati od inkubacije, praćen je utjecaj ovih mikroorganizama na ličinke velikog voskovog moljca (Galleria mellonella). Tretmani za ispitivanje insekticidnog djelovanja bioagensa su postavljeni s preporučenom (1*108) i dvostrukom koncentracijom (2*108) B. bassiana i B. pseudobassiana, te preporučenom koncentracijom (1*1013) B. subtilis. U laboratorijskim uvjetima također je utvrđen postotak radijalne inhibicije rasta fitopatogena (PRIRP) Fusarium oxysporum f. sp. lycopersici u interakciji s B. bassiana. Dvostruka koncentracija B. bassiana pokazala je 100% insekticidno djelovanje 24 sata nakon primjene, dok za B. subtilis nije utvrđeno insekticidno djelovanje. B. subtilis u kombiniranoj primjeni s azadiraktinom izaziva 100% mortalitet ličinki voskovog moljca, jednako kao i pojedinačna primjena azadiraktina. Ovim istraživanjem potvrđeno je insekticidno i antifungalno djelovanje B. bassiana, ali se preporučuju dodatna laboratorijska istraživanja s različitim sojevima B. bassiana kao i drugim vrstama fitopatogenih gljiva te različitih vrste štetnika kako bi se potvrdili ovi rezultati
Abstract (english)
Synthetic chemical pesticides are used as main tool in pest managements programs. However, due to their negative impact on human health and the environment, modern agricultural production requires the application of environmentally friendly control methods, which include entomopathogenic fungi and beneficial bacteria. The aim of this study was to determine the insecticidal and antifungal efficacy of bioagens in single and combined application of the entomopathogenic fungus Beauveria bassiana and the beneficial bacterium Bacillus subtilis. The study was conducted under laboratory conditions, and every 24, 48, 72, and 96 hours after incubation, the influence of these microorganisms on the larvae of the large wax moth (Galleria mellonella) was monitored. Treatments to test the insecticidal activity of the bioagent were set with the recommended (1*108) and double concentration (2*108) of B. bassiana and B. pseudobassiana, and the recommended concentration (1*1013) of B. subtilis. The percentage of radial growth inhibition of the phytopathogen (PRIRP) of Fusarium oxysporum f sp. lycopersici by B. bassiana was also determined under laboratory conditions. Double the concentration of B. bassiana showed 100% insecticidal activity 24 hours after application, while for B. subtilis no insecticidal activity was found. B. subtilis in combination with azadiractin causes 100% mortality of wax moth larvae, as well as solely azadiractin treatments. This study confirmed the insecticidal and antifungal activity of B. bassiana, but additional laboratory studies with different strains of B. bassiana as well as other species of phytopathogenic fungi and different types of pests are recommended to confirm these results.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:818668
Study programmeTitle: Plant Production; specializations in: Plant Production, Plant Nutrition and Soil Science, Plant Selection and Seed Production, Plant Protection
Course: Plant Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka bilinogojstva (magistar/ magistra inženjer/inženjerka bilinogojstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public notediplomski rad
Created on2021-10-12 06:52:58