Identifikacija Dreb-B1 lokusa u genotipovima vrsta Triticum spelta L. i Triticum aestivum L.

Tomić, Filip

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undergraduate thesis

Identifikacija Dreb-B1 lokusa u genotipovima vrsta Triticum spelta L. i Triticum aestivum L.

2022. urn:nbn:hr:151:074663

Tomić, Filip

Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

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APA 6th Edition

Tomić, F. (2022). Identifikacija Dreb-B1 lokusa u genotipovima vrsta Triticum spelta L. i Triticum aestivum L. (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:074663

MLA 8th Edition

Tomić, Filip. "Identifikacija Dreb-B1 lokusa u genotipovima vrsta Triticum spelta L. i Triticum aestivum L.." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:151:074663

Chicago 17th Edition

Harvard

Tomić, F. (2022). 'Identifikacija Dreb-B1 lokusa u genotipovima vrsta Triticum spelta L. i Triticum aestivum L.', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:151:074663

Vancouver

Tomić F. Identifikacija Dreb-B1 lokusa u genotipovima vrsta Triticum spelta L. i Triticum aestivum L. [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2022 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:151:074663

IEEE

F. Tomić, "Identifikacija Dreb-B1 lokusa u genotipovima vrsta Triticum spelta L. i Triticum aestivum L.", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:151:074663

Cite this item: https://urn.nsk.hr/urn:nbn:hr:151:074663

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Metadata

Title	Identifikacija Dreb-B1 lokusa u genotipovima vrsta Triticum spelta L. i Triticum aestivum L.
Title (english)	Identification of Dreb-B1 locus in Triticum spelta L. and Triticum aestivum L. genotypes
Author	Filip Tomić
Mentor	Sonja Petrović (mentor)
Committee member	Sonja Vila (predsjednik povjerenstva)
Committee member	Sunčica Kujundžić (član povjerenstva)
Granter	Josip Juraj Strossmayer University of Osijek Faculty of Agrobiotechnical Sciences Osijek (Department of Plant Production and Biotechnology) Osijek
Defense date and country	2022-09-28, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Agronomy Genetics and Breeding of Plants, Animals and Microorganisms
Abstract	Povećanjem svjetske populacije povećava se potreba za proizvodnjom visokorodnih kultivara koji će zadovoljiti potrebe za hranom. Proizvodnja uvelike ovisi o abiotskim čimbenicima te je često podvrgnuta stresnim uvjetima. Tijekom stresnih uvjeta DREB skupina proteina reagira ekspresijom mnogih gena čime se postiže poboljšana tolerancija na stresne uvjete poput suše, povećane količine soli te niskih temperatura. U istraživanju je sudjelovalo 13 genotipova iz vrsta Triticum gdje je bio cilj utvrditi prisutnost Dreb-B1 lokusa. Uspješno je izolirana DNA odgovarajuće čistoće i kvalitete, A260/A280 omjerom apsorbancija od 1,8 do 1,88. Provedena je PCR reakcija s početnicama P18R i P18L koje su specifične za Dreb-B1 gen koji se nalazi na 3B kromosomu pšenice. Utvrđena je prisutnost specifičnog alela koji karakterizira prisutnost Dreb-B1 gena kod četiri uzorka: T. diccocoides, T. spelta, T. sphaeroccum te kultivara Indigo. Nedostatak specifičnog alela na kromosomu 3B uočen je u šest genotipova: T. monococcum, T. compactum, te u kultivara Nirvana, Bc Tena, Moskva 40 i Sirban prolifik. Od ispitivanih 13 uzoraka na jednom uzorku, T. diccocoides (SLO), nije zabilježena amplifikacija.
Abstract (english)	Increasing the world's population increases the need for the production of high -growing cultivars that will meet food needs. Production depends largely on the abiotic factors and is often confirmed by stressful conditions. During stressful conditions, the DREB groups of protein respond to the expression of many genes, which achieves improved tolerance to stressful conditions such as drought, increased amounts of salt and low temperatures. The research was conducted on 13 genotypes from Triticum species, where it aimed to determine the presence of the Dreb-B1 locus. The DNA isolation was successfully isolated, with A260/A280 ratio 1.8 - 1.88. PCR reaction was carried out with SSRs P18R and P18L specific to the Dreb-B1 gene located on wheat chromosome 3B. The presence of a specific allele of Dreb-B1 gene was identified in four samples: T. diccocoides, T. spelta, T. sphaerococcum and Indigo. Lack of the specific allele on chromosome 3B was observed in six genotypes: T. monococcum, Nirvana, T. compactum, BC Tena, Moskva 40 and Sirban Prolifik. From the 13 samples tested, amplification was not successfully implemented in T. diccocoides.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:151:074663
Study programme	Title: Agriculture; specializations in: Agroeconomics, Plant production, Mechanization, Zootechnique, Horticulture Course: Plant production Study programme type: university Study level: undergraduate Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Public note	završni rad
Created on	2022-10-19 10:22:43