Management of the olive fly can be carried out by mechanical, chemical and biological measures. Due to the increasing demand for ecologically produced olive oil, and due to the increased pest resistance to chemical agents, biocontrol of olive fruit fly has been tested intensively. The aim of the work was to determine the average damage to olive fruits by the olive fly (Bactrocera oleae), and the influence of different concentrations of azadirachtin and entomopathogenic nematodes (Steinernema feltiae) on the pupae of this pest. Olive fruits were collected from olive groves in September 2021 from the area of southern Dalmatia. The damage on the skin of the fruit in the form of darker spots, lesions or dried areas that occurs after the oviposition of the olive fly, i.e. the activity of the fly larvae, was assessed. The larvae or pupae of the olive fly were separated, weighed and counted. The damage to the olive fruits ranged up to a maximum of 30% of the olive fruits examined, and it depended mostly on the location of collected olive fruits. Thus, on the locations on the island of Hvar, the greatest damage to the olive fruit was determined. The laboratory experiment was carried out on olive fly pupae that were placed in moist vermiculite. Vermiculite was treated with four different concentrations of azadirachtin (OzoNeem), an entomopathogenic nematode Steinernema feltiae, with positive (Actara 25%, thiamethoxam) and negative (water) controls. All treatments had a significant effect on the development of pupa i.e on the appearance of the adult stages of the olive fly. Compared to the negative control, the treatment with entomopathogenic nematodes was the most effective. Compared to chemical insecticides, research results show that azadirachtin and entomopathogenic nematodes are good solutions for olive fly control, and their inclusion in integrated olive protection methods is recommended. This research confirms the effectiveness of ecologically acceptable methods of olive protection, reducing the use of chemicals in agriculture.