| Abstract | U disertaciji se istražuju postupci obogaćivanja konzumnih jaja s n-3 polinezasićenim masnim kiselinama i utjecaj obogaćenih jaja na ljudsko zdravlje. Za istraživanje je upotrebljeno 480 TETRA SL nesilica koje su podijeljene u tri skupine (tretmani K, P1 i P2). Smjesa tretmana K bila je kontrolna i sadržavala je 5% sojinog ulja. Smjese tretmana P1i P2 sadržavale su umjesto sojinog ulja kombinaciju lanenog i ribljeg ulja 3,5% + 1,5%, odnosno 3% i 2%. Utvrđene su značajne razlike (P=0,001) između Σn-3 PUFA kao i omjera Σn-6/n-3 PUFA između kontrolne i pokusnih smjesa za nesilice (P=0,001). Kontrolna smjesa za nesilice sadržavala je 5,04%, P1 smjesa 33,86%, a P2 smjesa 33,59% Σ n-3 PUFA. Omjer Σn-6/ Σn-3 PUFA iznosio je u kontrolnoj smjesi 10,28:1, P1 smjesi 0,69:1 i P2 smjesi 0,68:1. Profili masnih kiselina u lipidima jaja ovisili su o hranidbenim tretmanima. Rezultati analize sadržaja ALA, EPA i DHA pokazuju da su jaja P1 i P2 skupina značajno obogaćena s n-3 PUFA u odnosu na K skupinu jaja (P=0,001) i to: ALA (5,6 % i 5,2%), EPA (0,4% i 0,4%) i DHA (2,8% i 2,7%), a sve bez negativnog utjecaja na pokazatelje kvalitete jaja. Profili n-3 PUFA u krvi ispitanika pri konzumaciji konvencionalnih i obogaćenih jaja (tretmani K i P1) statistički su se značajno razlikovali u ΣSFA (P=0,041) i sadržaju ALA (P=0,010). Konzumacijom n-3 PUFA obogaćenih jaja ostvareno je smanjenje omjera Σn-6 PUFA/Σn-3 PUFA u serumu ispitanika (27%) kao i povoljan utjecaj na određen biokemijske pokazatelje. |
| Abstract (english) | The dissertation examines the processes of enriching table eggs with n-3 polyunsaturated fatty acids and the impact of enriched eggs on human health. For the research, 480 TETRA SL layers were used, which were divided into three groups (treatments K, P1 and P2). The K treatment mixture was controlled and contained 5% soybean oil. Treatment mixtures P1 and P2 contained instead of soybean oil a combination of linseed oil and fish oil 3.5% + 1.5%, respectively 3% and 2%. Significant differences (P=0.001) were found between Σn-3 PUFA as well as Σn-6/n-3 PUFA ratio between control and experimental mixture for laying hens (P=0.001). The control mixture for layers contained 5.04%, P1 mixture 33.86%, and P2 mixture 33.59% Σn-3 PUFA. The ratio of Σn-6/ Σn-3 PUFA was 10.28:1 in the control mixture, 0.69:1 in the P1 mixture and 0.68:1 in the P2 mixture. Fatty acid profiles in egg lipids depended on feeding method. The results of the analysis of the content of ALA, EPA and DHA show that eggs of groups P1 and P2 are significantly enriched in n-3 PUFA compared to eggs of group K (P=0.001), namely: ALA (5.6% and 5.2%), EPA (0.4% and 0.4%) and DHA (2.8% and 2.7%), all without negative impact on egg quality indicators. The n-3 PUFA profiles in the blood of subjects who consumed conventional and enriched eggs (treatments K and P1) were statistically significantly different in the content of Σ SFA (P=0.041) and ALA (P=0.010). The consumption of eggs enriched with n-3 PUFA resulted in a decrease in the ratio of Σ n-6 PUFA/Σ n-3 PUFA in the serum of the subjects (27%), as well as a favorable effect on certain biochemical indicators. |
