Survival and viability of Bradyrhizobium japonicum in different liquid medium

Kanižai Šarić, Gabriella; Majić, Ivana; Prtenjača, Krešimir

doi:10.33128/ag.82.5-6.2

Scientific paper - Original scientific paper

Survival and viability of Bradyrhizobium japonicum in different liquid medium

Agronomski glasnik : Glasilo Hrvatskog agronomskog društva, 82 (2021), 5-6; 245-252. https://doi.org/10.33128/ag.82.5-6.2

Kanižai Šarić, Gabriella; Majić, Ivana; Prtenjača, Krešimir

Cite this document

APA 6th Edition

Kanižai Šarić, G., Majić, I. & Prtenjača, K. (2021). Survival and viability of Bradyrhizobium japonicum in different liquid medium. Agronomski glasnik : Glasilo Hrvatskog agronomskog društva, 82. (5-6), 245-252. doi: 10.33128/ag.82.5-6.2

MLA 8th Edition

Kanižai Šarić, Gabriella, et al. "Survival and viability of Bradyrhizobium japonicum in different liquid medium." Agronomski glasnik : Glasilo Hrvatskog agronomskog društva, vol. 82, no. 5-6, 2021, pp. 245-252. https://doi.org/10.33128/ag.82.5-6.2

Chicago 17th Edition

Kanižai Šarić, Gabriella, Ivana Majić and Krešimir Prtenjača. "Survival and viability of Bradyrhizobium japonicum in different liquid medium." Agronomski glasnik : Glasilo Hrvatskog agronomskog društva 82, no. 5-6 (2021): 245-252. https://doi.org/10.33128/ag.82.5-6.2

Harvard

Kanižai Šarić, G., Majić, I. and Prtenjača, K. (2021) 'Survival and viability of Bradyrhizobium japonicum in different liquid medium', Agronomski glasnik : Glasilo Hrvatskog agronomskog društva, 82(5-6), pp. 245-252. doi: 10.33128/ag.82.5-6.2

Vancouver

Kanižai Šarić G, Majić I, Prtenjača K. Survival and viability of Bradyrhizobium japonicum in different liquid medium. Agronomski glasnik : Glasilo Hrvatskog agronomskog društva [Internet]. 2021 September 30 [cited 2024 December 19];82(5-6):245-252. doi: 10.33128/ag.82.5-6.2

IEEE

G. Kanižai Šarić, I. Majić and K. Prtenjača, "Survival and viability of Bradyrhizobium japonicum in different liquid medium", Agronomski glasnik : Glasilo Hrvatskog agronomskog društva, vol. 82, no. 5-6, pp. 245-252, September 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:151:678573. [Accessed: 19 December 2024]

Cite this item: https://urn.nsk.hr/urn:nbn:hr:151:678573

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Metadata

Title (english)	Survival and viability of Bradyrhizobium japonicum in different liquid medium
Author	Gabriella Kanižai Šarić
Author	Ivana Majić
Author	Krešimir Prtenjača
Author's institution	Josip Juraj Strossmayer University of Osijek Faculty of Agrobiotechnical Sciences Osijek (Department of agroecology and Enviroment Protection)
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Agronomy Ecology and Environmental Protection
Abstract (english)	The microbiological inoculants present on the market come in various formulations and forms. Inoculants used in pre-sowing bacterization of legumes in our country are traditionally prepared on peat as the highest quality carrier of bacteria. However, the requirements of the manufacturer are focused on liquid forms of inoculants. Therefore, the aim of this paper is to determine the optimal composition of the liquid medium that will support the growth of Bradyrhizobium japonicum, the soybean symbiont. Three liquid nutrient medium formulations were included in the study: mannitol-yeast medium, modified mannitol-yeast medium, and glycerol medium stored at two temperatures (4 and 25 °C), and B. japonicum cell viability was determined over 6 months. The results showed that the largest number of rhizobia (on average 4x109 zo 9x108) was obtained on yeast-mannitol medium at 4 °C as well as on modified yeast mannitol medium where their number remained constant throughout the storage time and was 5x107 cfu ml-1 at 25 °C. Further research should include testing other rhizobial protectors in order to increase the number of viable cells in longer time periods.
Keywords (english)
Language	english
Publication type	Scientific paper - Original scientific paper
Publication status	Published
Peer review	Peer review - domestic
Publication version	Published version
Journal title	Agronomski glasnik : Glasilo Hrvatskog agronomskog društva
Numbering	vol. 82, no. 5-6, pp. 245-252
p-ISSN	0002-1954
e-ISSN	1848-8900
DOI	https://doi.org/10.33128/ag.82.5-6.2
URN:NBN	urn:nbn:hr:151:678573
Publication	2021-09-30
Document URL	https://hrcak.srce.hr/264684
Type of resource	Text
Access conditions	Open access
Terms of use
Created on	2024-05-23 12:13:04