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master's thesis
Primjena efektivnih mikroorganizama u fazi rizogeneze i aklimatizacije borovnice ( Vaccinium corymbosum L.) in vitro
2024. urn:nbn:hr:151:458880
Bižić, Tomislav
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

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Bižić, T. (2024). Primjena efektivnih mikroorganizama u fazi rizogeneze i aklimatizacije borovnice ( Vaccinium corymbosum L.) in vitro (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:458880

Bižić, Tomislav. "Primjena efektivnih mikroorganizama u fazi rizogeneze i aklimatizacije borovnice ( Vaccinium corymbosum L.) in vitro." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2024. https://urn.nsk.hr/urn:nbn:hr:151:458880

Bižić, Tomislav. "Primjena efektivnih mikroorganizama u fazi rizogeneze i aklimatizacije borovnice ( Vaccinium corymbosum L.) in vitro." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2024. https://urn.nsk.hr/urn:nbn:hr:151:458880

Bižić, T. (2024). 'Primjena efektivnih mikroorganizama u fazi rizogeneze i aklimatizacije borovnice ( Vaccinium corymbosum L.) in vitro', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 14 December 2024, https://urn.nsk.hr/urn:nbn:hr:151:458880

Bižić T. Primjena efektivnih mikroorganizama u fazi rizogeneze i aklimatizacije borovnice ( Vaccinium corymbosum L.) in vitro [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2024 [cited 2024 December 14] Available at: https://urn.nsk.hr/urn:nbn:hr:151:458880

T. Bižić, "Primjena efektivnih mikroorganizama u fazi rizogeneze i aklimatizacije borovnice ( Vaccinium corymbosum L.) in vitro", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:151:458880

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TitlePrimjena efektivnih mikroorganizama u fazi rizogeneze i aklimatizacije borovnice ( Vaccinium corymbosum L.) in vitro
Title (english)Application of effective microorganisms in the phase of rhizogenesis and acclimatization of blueberry (Vaccinium corymbosum L.) in vitro
AuthorTomislav Bižić
MentorDejan Bošnjak (mentor)
Committee memberAleksandar Stanisavljević (predsjednik povjerenstva)
Committee memberMonika Tkalec Kojić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Plant Production and Biotechnology)
Osijek
Defense date and country2024-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Fruit Growing
Abstract
Efektivni mikroorganizmi (EMO) predstavljaju mješovite kulture korisnih mikroorganizama, a njihova primjena u kulturi biljnog tkiva in vitro nije uobičajena niti široko istražena. Na Fakultetu agrobiotehničkih znanosti Osijek (FAZOS) u okviru laboratorija za voćarstvo, provedeno je istraživanje s ciljem ispitivanja primjene EMO tjekom faza rizogeneze i aklimatizacije borovnice ( Vaccinium corymbosum L.) prilikom prijelaza iz in vitro u ex vitro uvjete. Rezultati ovog istraživanja pokazali su značajne razlike u stopama preživljavanja između primjenjenih tretmana i kultivara nakon aklimatizacije: Najveća stopa preživljavanja zabilježena je kod kultivara Duke ( 43,4%), dok su Bluecrop (18%) i Lagacy (8,7%) imali znatno niže stope. Tretman T2 (Trichoderma koningii i Trichoderma horzianum) pokazao se kao najdjelotvorniji, s 32 % preživjelih izdanaka, nadmašivši kontrolu (29,3%), dok su ostali tretmani rezultirali nižim stopama preživljavanja. Nisu pronađene statistički značajne razlike u duljini izdanaka, broju korjenčića ili duljini korjenčićaa između tretmana, osim u broju listova (> 0,5 cm), gdje je kontrola pikazala bolje rezultate. Iako Trichoderma spp. nije imala značajan utjecaj na broj i duljinu korjenčića, tretman T2 dao je bolje rezultate s tim parametrima u usporedbi s ostalim tretmanima: Visoke koncentracije EMO i NAA vjerojatno su imale toksičan učinak na kultivare Bluecrop i Lagacy, uzrokujući posmeđenje i odumiranje izdanaka, osobito kod kultivara Legacy. Potrebna su daljnja istraživanja s različitim koncentracijama EMO i NAA kako bi se ovi učinci bolje razumjeli.
Abstract (english)
Effective microorganisms (EMO) represent mixed cultures of beneficial microorganisms, and their application in in vitro plant tissue culture is neither common nor widaly researched. at the Faculty of Agrobiotechnical Sciences Osijek (FAZOS), within the fruit growing laboratory, a study was conducted with the aim of examinning the application of EMO during the rhizogenesis and acclimatization phases of blueberry (Vaccinium corymbosum L.) from in vitro to ex vitro conditions. The results of this study revealed significant differences in survival rates between the applied treatments and cultivars after acclimatization. The highest survival rate was recorded in the Duke cultivar (43,4%), while Bluecrop (18%)and Legacy (8,7%) exhibited significently lower rates. Treatment T2 (Trichoderma koningii and Trichoderma harzianum) proved to be the most effective, with 32 % of surviving shoots, outperforming the control (29,3%), while other treatments resulted in lower survival rates. No statistically significant differences were found in shoot langth, root number, or root number, or root length between treatments, except inthe number of leaves (>0,5 cm), where the control showed better results. Although Trichoderma spp. did not have a significant effect on root number and langth, treatment T2 showed better results in these parameters compared to other treatments. High concentration of EMO and NAA likely had a toxic effect onthe Bluecrop and Legacy cultivars, causing browning and shoot dieback, particulary in the Legacy cultivar. Further research with different concentrations of EMO and NAA is needed to better understand these effests.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:458880
Study programmeTitle: Fruit growing, viticulture and wine; specializations in: Fruit growing, Viticulture
Course: Fruit growing
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka voćarstva, vinogradarstva i vinarstva (magistar/ magistra inženjer/inženjerka voćarstva, vinogradarstva i vinarstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Public notediplomski rad
Created on2024-11-28 16:03:24