The research component of this thesis was conducted in the Plant Tissue Culture Laboratory, Department of Pomology, Viticulture, and Enology ( Pomology Laboratory) at the Faculty of Agrobiotechnical Sciences Osijek (FAZOS). The aim of this thesis was examine the effects of silicon dioxide (Sio2) nanoparticles on the anatomical characteristics of blueberry (Vaccinium corymbosum L.) leaves, specifically the size and number of stomata, within the SETIS liquid immersion bioreactor system. Although the statistical were not significant, stomata in treatments with nanoparticles , especially with the lower concentration of Sio2 nanoparticles (30 mg/l) exhibited larger dimensions and pore openings compared to the control traetment. The results suggest a potential effect, primarily of the lower concentations (30mg/L) but also of the higher concentration (75 mg/L) of SiO2 nanoparticles on the anatomical features of stomata (size and pore openings) in blueberry leaves grown in the immersion bioreactor system. SiO2 nanoparticles, at both concentrations ( 30 and 75 mg/L) positively influenced the anatomical characteristics of stomata, as in vitro shoots in both treatments developed larger and more robust stomata compared to the control treatment without nanoparticles. By the end of the 45-day cycle, the plant material had developed an ideal biomass and showed no signs of stress, severe vitrification, or any negative effects from the applied concentrations of SiO2 nanoparticles. Visual analysis also revealed greater shoot height and an increased number of shoots in nanoparticle-treated plants. which developed more and greener leaves compared to the control treatment. The effect of the applied Sio2 nanoparticle concentrations on the development of a greater number of stomata per mm2 of epidrmal tissue in blueberry explants in the liquid immersion system was not confirmed. The results of our research emphasize the potential and value of applying SiO2 nanoparticles in plant biotechnology, particularly in improving the efficiency of in vitro propagation methods within the liquid immersion bioreactor system. Future research should focus on testing other nanoparticle concentrations and exploring the mechanisms underlying the observed effects, as well as optimizing their application to other plant, especially fruit species.