The aim of this study was to present the performance and some biochemical parameters in the blood of hens fed feed mixtures with different levels of lutein. The study involved a total of 291 Tetra SL hybrid laying hens, divided into 3 groups (O0 with 0 mg/kg lutein added, O200 with 200 mg/kg lutein added and O400 with 400 mg/kg lutein added), and lasted for 5 weeks. The O0 group included 93 hens, whereas experimental groups 98 and 100 hens, respectively. The highest egg production and the best feed consumption per egg were recorded in O400 group with a total of 3367 pieces of eggs produced and 128.60 g of feed consumed. The best laying intensity (96.68%) and number of eggs per laying hen (34.19) as well as the highest feed consumption per day (129.01 g), was recorded in O200 group. Lutein added to feed mixtures for laying hens did not significantly affect the values of biochemical parameters in blood (P>0.05). An increase in total cholesterol (CHOL) and HDL cholesterol content as well as the decrease of total protein (PROT) and albumin (ALB) content in the experimental groups can be observed. The content of glucose (GUK) and urea was similar between the experimental groups, while the highest content of triglycerides (TGC) was recorded in O200 group. It can be concluded that different levels of lutein added to the feed mixtures for laying hens did not have negative effect on the performance and blood biochemical parameters of laying hens.