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Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis
Croatica Chemica Acta, 84 (2011), 2; 259-268. https://doi.org/10.5562/cca1808
Špoljarić, Jasminka; Tomić, Antonija; Vukelić, Bojana; Salopek-Sondi, Branka; Agić, Dejan; Tomić, Sanja; Abramić, Marija

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Špoljarić, J., Tomić, A., Vukelić, B., Salopek Sondi, B., Agić, D., Tomić, S. i Abramić, M. (2011). Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis. Croatica Chemica Acta, 84. (2), 259-268. doi: 10.5562/cca1808

Špoljarić, Jasminka, et al. "Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis." Croatica Chemica Acta, vol. 84, br. 2, 2011, str. 259-268. https://doi.org/10.5562/cca1808

Špoljarić, Jasminka, Antonija Tomić, Bojana Vukelić, Branka Salopek Sondi, Dejan Agić, Sanja Tomić i Marija Abramić. "Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis." Croatica Chemica Acta 84, br. 2 (2011): 259-268. https://doi.org/10.5562/cca1808

Špoljarić, J., et al. (2011) 'Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis', Croatica Chemica Acta, 84(2), str. 259-268. doi: 10.5562/cca1808

Špoljarić J, Tomić A, Vukelić B, Salopek Sondi B, Agić D, Tomić S, i sur.. Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis. Croatica Chemica Acta [Internet]. 03.10.2011. [pristupljeno 12.07.2024.];84(2):259-268. doi: 10.5562/cca1808

J. Špoljarić, et al., "Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis", Croatica Chemica Acta, vol. 84, br. 2, str. 259-268, Listopad 2011. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:151:172807. [Citirano: 12.07.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:151:172807
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Naslov (engleski)Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis
AutorJasminka Špoljarić
AutorAntonija Tomić
AutorBojana Vukelić
AutorBranka Salopek-Sondi
AutorDejan Agić
AutorSanja Tomić
AutorMarija Abramić
Autorova ustanovaSveučilište Josipa Jurja Strossmayera u Osijeku
Fakultet agrobiotehničkih znanosti Osijek
(Zavod za agroekologiju i zaštitu okoliša)
Znanstveno / umjetničko
područje, polje i grana		BIOTEHNIČKE ZNANOSTI
Poljoprivreda (agronomija)
Agrokemija
Sažetak (engleski)
Human dipeptidyl peptidase III (DPP III) is a member of the zinc-metallopeptidase family M49 with a role in intracellular protein catabolism. Ligand-free crystal structure of yeast and human orthologues provided insight into the enzyme's active center and enabled some assumptions about the enzyme - substrate interactions. The molecular modeling performed for human and yeast DPP III indicated involvement of Asn406 and Asn415, respectively in ligand binding. To investigate further the role of this asparagine residue, conserved in all eukaryotic M49 peptidases, a site-directed mutagenesis of human DPP III was carried out. Replacement of Asn406 with Gln, but not with Ser, resulted in a large decrease in the enzyme's binding affinity for competitive peptide inhibitor and in catalytic efficiency. Molecular dynamics simulations performed for enzyme-substrate complexes, revealed changed coordination of the active- site zinc ion and the orientation of the catalytically important His568, in N406Q, but not in the N406S complexes.(doi: 10.5562/cca1808)
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - domaća recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCroatica Chemica Acta
Brojčani podacivol. 84, br. 2, str. 259-268
p-ISSN0011-1643
e-ISSN1334-417X
DOIhttps://doi.org/10.5562/cca1808
URN:NBNurn:nbn:hr:151:172807
Datum objave publikacije2011-10-03
URL dokumentahttps://hrcak.srce.hr/71989
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2024-07-04 06:20:52