The research aimed to determine the blood metabolic profile and acid-base status of lactating Travnik ewes in an extensive production system. The research was carried out on 108 lactating Travnik ewes, with an average age of 3-4 years, in their 3rd lactation. The ewes were kept on pasture, and had water and animal salt ad libitum. Hematological indicators were determined in whole blood, biochemical parameters in serum, and acid-base status was determined in plasma. The determined average values of hematological parameters were mostly within reference values, except the lower MCHC content and higher MCV content, which indicates the instability of erythrocyte constants. Average mineral concentrations were within the reference values, except for Ca and Fe concentrations which were lower and were influenced by higher milk excretion. Most of the biochemical indicators, enzyme activity, and indicators of the acid-base balance of lactating Travnik ewes’ blood were within the reference values. A high concentration of urea above the reference values was found, and concentrations of total proteins, albumins, total cholesterol, HDL and LDL cholesterol, and triglycerides at the upper limit or above the reference values were determined. Slightly lower GPx activity and higher SOD activity above reference values were determined. These indicators point to energy deficit, as well as poor grazing quality, and a lack of selenium in pasture plant species. When determining the blood metabolic profile of lactating Travnik ewes, the obtained results of the research should be considered, and they should be included in the development of reference values for the Travnik sheep breed.