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undergraduate thesis
Izolacija genomske DNA iz zrna pšenice
2019. urn:nbn:hr:151:129560
Stilin, Anamarija
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechical Sciences Osijek
Department of Plant Production and Biotechnology

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Stilin, A. (2019). Izolacija genomske DNA iz zrna pšenice (Undergraduate thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:129560

Stilin, Anamarija. "Izolacija genomske DNA iz zrna pšenice." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:151:129560

Stilin, Anamarija. "Izolacija genomske DNA iz zrna pšenice." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:151:129560

Stilin, A. (2019). 'Izolacija genomske DNA iz zrna pšenice', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek, accessed 28 February 2021, https://urn.nsk.hr/urn:nbn:hr:151:129560

Stilin A. Izolacija genomske DNA iz zrna pšenice [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek; 2019 [cited 2021 February 28] Available at: https://urn.nsk.hr/urn:nbn:hr:151:129560

A. Stilin, "Izolacija genomske DNA iz zrna pšenice", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:151:129560

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Metadata
TitleIzolacija genomske DNA iz zrna pšenice
Title (english)Genomic DNA extraction from wheat grain
AuthorAnamarija Stilin
MentorSonja Petrović (mentor)
Committee memberSonja Vila (predsjednik povjerenstva)
Committee memberAndrijana Rebekić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechical Sciences Osijek
(Department of Plant Production and Biotechnology)
Osijek
Defense date and country2019-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Genetics and Breeding of Plants, Animals and Microorganisms
Abstract
Pšenica je iza kukuruza druga najznačajnija žitarica iz porodice trava. Cilj ovog rada bio je uspješno izolirati genomsku DNA iz mljevenog zrna pšenice bez primjesa. Samo istraživanje provelo se u dvije faze: u prvoj fazi provedene su dvije metode izolacije DNA iz zrna pšenice, a to su modificirana CTAB metoda s dodatkom PVP i SDS metoda, a DNeasy® Plant Mini Kit test korišten je kao brza provjera kvalitete DNA . Druga faza sastoji se od spektrofotometrijske analize izolirane DNA. Modificirana CTAB metoda se pokazala kao najuspješnija s optimalnom čistoćom i koncentracijom izolirane DNA (A260/280 2,00 – 1,99, A260/230 2,19 – 2,34, koncentracija 97,10 – 181 ng/μL).
Abstract (english)
The wheat besides corn is the world the second most important crop of the grass family. The aim of this work was to successfully isolate genomic DNA from milled wheat grains without secondary methabolutes. The research was carried out in two phases: in the first phase were used two DNA isolation methods: modified CTAB method with PVP supplement and SDS method, and the DNeasy® Plant Mini Kit test was used as a quick DNA quality check. Second phase consist of spectrophotometric determination isolated DNA. Modified CTAB method was most successful generating optimal DNA quality and quantity results (A260/280 2.00 – 1.99, A260/230 2.19 – 2.34, concentration 97.10 – 181 ng/μL).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:129560
Study programmeTitle: Agriculture; specializations in: Agroeconomics, Plant production, Mechanization, Zootechnique, Horticulture
Course: Horticulture
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public notezavršni rad
Created on2019-09-30 08:45:14