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undergraduate thesis
Digestija genomske DNA restrikcijskim endonukleazama
2022. urn:nbn:hr:151:726622
Kovač, Jelena
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

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Kovač, J. (2022). Digestija genomske DNA restrikcijskim endonukleazama (Undergraduate thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:726622

Kovač, Jelena. "Digestija genomske DNA restrikcijskim endonukleazama." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:151:726622

Kovač, Jelena. "Digestija genomske DNA restrikcijskim endonukleazama." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:151:726622

Kovač, J. (2022). 'Digestija genomske DNA restrikcijskim endonukleazama', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 21 January 2023, https://urn.nsk.hr/urn:nbn:hr:151:726622

Kovač J. Digestija genomske DNA restrikcijskim endonukleazama [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2022 [cited 2023 January 21] Available at: https://urn.nsk.hr/urn:nbn:hr:151:726622

J. Kovač, "Digestija genomske DNA restrikcijskim endonukleazama", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:151:726622

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Metadata
TitleDigestija genomske DNA restrikcijskim endonukleazama
Title (english)Restriction endonuclease digestion of genomic DNA
AuthorJelena Kovač
MentorSonja Petrović (mentor)
Committee memberSonja Vila (predsjednik povjerenstva)
Committee memberSunčica Kujundžić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Plant Production and Biotechnology)
Osijek
Defense date and country2022-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Genetics and Breeding of Plants, Animals and Microorganisms
Abstract
Restrikcijska endonukleaza je naziv za enzim pronađen u bakterijskim stanicama kao dio njihova restrikcijsko modifikacijskog sustava koji im služi u obrani i razgradnji virusne DNA. Cilj ovog istraživanja je bio izolirati genomsku DNA iz tri biljne vrste s različitom veličinom genoma te provesti uspješnu digestiju genomske DNA restrikcijskim endonukleazama. U radu su korištene vrste Brachypodium distachyon L. i kukruz (Zea mays L.) i šest restrikcijskih endonukleaza. Genomska DNA izolirana je koristeći CTAB metodu, a digestija izolirane DNA je optimizirana te su rezultati cijepanja provjeren elektroforezom. Utvrđeno je da su najpogodniji enzimi za cijepanje genosmke DNA Brachypodium distachyon L., EcoRI i BamHI, a za Zea mays L. to su enzimi HindIII, RspRSII, EcoRI, pri uvjetima inkubacije i volumenu sastavnica restrikcijskih smjesa propisanih od strane proizvođača.
Abstract (english)
Restriction endonuclease is the name for an enzyme found in bacterial cells as part of their restriction modification system that are used in defense and degradation of foreign DNA. The aim of the study was to isolate genomic DNA from three plant species with different genome sizes and to perform successful digestion of genomic DNA with restriction endonucleases. The species Brachypodium distachyon L. and maize (Zea mays L.) and six restriction endonucleases were used in the work. Genomic DNA was isolated using the CTAB method, digestion of the isolated DNA was optimized and the cleavage results were verified by electrophoresis. It was determinedthat the most suitable enzymes for genomic DNA of Brachypodium distachyon L. are EcoRI and BamHI, and for Zea mays L. to su enzimi HindIII, RspRSII, EcoRI, under the conditions of incubation and the volume of components of the restriction mixtures prescribed by the manufacturer.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:726622
Study programmeTitle: Agriculture; specializations in: Agroeconomics, Plant production, Mechanization, Zootechnique, Horticulture
Course: Horticulture
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2022-10-19 09:29:03