The aim of this study was to determine the total concentration of the water-soluble fractions of heavy metals in soils the Pannonian Basin. The study was conducted at six different areas Croatian, Bosnia and Herzegovina and Serbia. Significant differences in the concentrations of certain heavy metals were found on the different areas, and at different localities of particular areas. It was established that are higher concentrations of heavy metals in analyzed soil in forms that are less accessible to plants, while is in soil solution of a minor fraction (water soluble fraction). This is particularly expressed with zinc, whose concentration in the aqueous solution was been a very low. Analysis of agrochemical properties of soil was established that are the soil of Prud and Banja Luka very low pH values. Exactly on this soils were are established higher concentrations of water-soluble fraction heavy metals. Established concentrations of nickel in the soil area of Banja Luka and Prud are 300% a maximum allowable amount. In other soils are concentration of heavy metal less than a maximum permissible concentration. We can conclude that are the soils of certain areas Pannonian basin elevated concentrations of heavy metals, but are not contaminated, and are suitable for agricultural production. The exceptions are the soils Banja Luka and Prud that need repairs, primarily neutralization of redundant acidity, which will reduce the water-soluble fraction of heavy metals.