This research examined the genetic diversity of soybean germplasm based on agronomical and morphological characteristic on set of 74 genotypes. Main objectives were to determine: (1) values of morphological traits for determining genotype distinctness criteria in the DUS testing procedure for registration and protection of new varieties, (2) to assess genetic variability of soybean germplasm based on morphological and agronomical characteristics, and (3) identify genotypes with favourable agronomic traits and select superior ones for future breeding programs. Field trials were set up in randomized complete block design with two replications at the Croatian Agency for Agriculture and Food, Centre for Seed and Seedlings during two growing seasons. During each year of the study, 19 morphological characteristics were analysed according to the UPOV soybean test guideline and seven agronomical traits. Analysed data showed that the greatest contribution to the determination of distinctness and the best differentiation of genotypes have the characteristics of beginning of flowering, time of maturity, colour of hairs of stem, flower colour or anthocyanin coloration of hypocotyl and plant height. The discriminant analysis showed that in dry seasons the proportions of maturity for later groups were less determined, while in moderate seasons the level of differentiation for earlier groups was less determined. The average diversity index for the morphological traits on the observed population of 74 genotypes was 0.77, which indicates a high degree of morphological diversity. The analysis of variance showed that genotype, year, and genotype x year interaction had a significant effect on all studied traits. By analysing agromorphological characteristics for determining genetic distance, grouping of genotypes did not follow the pattern of geographical origin, and the largest genetic distance was determined for pairs Paradis and Sapporo, using these combinations is most likely to increase the overall genetic variability of the soybean population