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undergraduate thesis
UTJECAJ RAZLIČITIH METODA NA KVALITETU IZOLIRANE DNA
2013. urn:nbn:hr:151:166320
Brica, Marina
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

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Brica, M. (2013). UTJECAJ RAZLIČITIH METODA NA KVALITETU IZOLIRANE DNA (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:166320

Brica, Marina. "UTJECAJ RAZLIČITIH METODA NA KVALITETU IZOLIRANE DNA." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2013. https://urn.nsk.hr/urn:nbn:hr:151:166320

Brica, Marina. "UTJECAJ RAZLIČITIH METODA NA KVALITETU IZOLIRANE DNA." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2013. https://urn.nsk.hr/urn:nbn:hr:151:166320

Brica, M. (2013). 'UTJECAJ RAZLIČITIH METODA NA KVALITETU IZOLIRANE DNA', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:151:166320

Brica M. UTJECAJ RAZLIČITIH METODA NA KVALITETU IZOLIRANE DNA [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2013 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:151:166320

M. Brica, "UTJECAJ RAZLIČITIH METODA NA KVALITETU IZOLIRANE DNA", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:151:166320

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Metadata
TitleUTJECAJ RAZLIČITIH METODA NA KVALITETU IZOLIRANE DNA
Title (english)INFLUENCE OF DIFFERENT DNA EXTRACTION METHODS ON DNA QUALITY
AuthorMarina Brica
MentorSonja Petrović (mentor)
Committee memberSonja Marić
Committee memberVlado Guberac
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Plant Production and Biotechnology)
Osijek
Defense date and country2013-10-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Crop Production
Abstract
Deoksiribonukleinska kiselina, DNA je genetski, nasljedni materijal prisutan u stanicama organizama svih živih bića, od najjednostavnijih do najsloženijih. Obično je vrlo teško izdvojiti i pročistiti kvalitetnu DNA iz žitarica zbog pojave polisaharida, proteina i DNA, kao inhibitora polimeraze kao što su tanini, alkaloidi, te polifenoli u ekstraktima. Ciljevi ovoga istraživanja bili su utvrditi kvalitetu i koncentraciju izolirane DNA iz biljke pšenice koristeći tri različite metode izolacije. Kvaliteta izoliranih DNA izmjerena je na spektrofotometru, tako što su izmjerene apsorbance na 260 i 280 nm. Omjeri absorbanci (A260/A280) su se kretali u rasponu od 1,92 (CTAB metoda), do 1,73 (BIOBASIC metoda). Najveća koncentracija DNA dobivena je primjenom SDS metode i to 2800 ng/μl, zatim 2280 ng/μl primjenom CTAB metode i najmanja koncentracija je dobivena BIOBASIC metodom (482,5 ng/μl). Sve tri metode su se pokazale učinkovitima za izolaciju DNA iz lista biljke pšenice te se mogu koristiti ovisno o svrsi daljnjeg istraživanja.
Abstract (english)
Deoxyribonucleic acid, DNA is the genetic hereditary material is present in cells of all living beings, from the simplest to the most complex. Usually it is very difficult to extract and purify high quality DNA from cereal because of presence of polysaccharides and proteins, as well as polymerase inhibitors such as tannins, alkaloids and polyphenols. The objective of this study was to determine the quality and quantity of the extracted isolated DNA from wheat seedlings using three different extraction methods, CTAB, SDS and a BIOBASIC commercial kit. The purity of the extracted DNA was measured by spectrophotometer using the absorbance at 260 and 280 nm. Absorbance ratio (A260/A280) ranged from 1.92 for CTAB method, to 1.73 for BIOBASIC method. The highest concentration of DNA was obtained using the SDS method, which yielded 2800 ng/μl, then 2280 ng/μl using CTAB method, and the lowest concentration was obtained by BIOBASIC method (482.5 ng/μl). All DNA extraction methods proved effective for DNA extraction from wheat seedlings and can be used depending on the purpose of further research.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:166320
Study programmeTitle: Agriculture; specializations in: Agroeconomics,Plant production, Mechanization, Zootechnique, Horticulture
Course: Plant production
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije)
Type of resourceText
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Created on2016-03-01 12:34:02