Objective of this study was to determine , based on results of agrochemical analisis of the soil and biological methods, ability of soil for the nitrogen mineralization. Laboratorical experiment was conducted with soil examples during 4 weeks. Potential of the nitrogen mineralization is stated for 30 soil samples with different content of humus by measuring concentration of nitrate nitrogen int he soil. Samples are diveded into 2 groups according to content of humus: on poor-humus soils and humus soils. Relation between content of total nitrogen int he soil, content mineralized nitrogen in the soil and intensity of soil-breathing with stated level of mineralized nitrogen in nitrate form after 28 days of incubation is also analized. Total level of mineralized nitrogen in the nitrate form has showed rapid increase after 7 days of incubation. Based on matemathical average of all 30 samples, average daily increase of nitrate nitrogen concetration in mineral form in first 7 days was 2,81 mg/kg/per day. In relative sum increase after 7 days of incubation was 168,5 % in relation to initial state. Althoug int he absolute sum the concetration oft he nitrate nitrogen in mineralized form was increasing during the four weeks of incubation, in relative sum for every other week there has been gradual decrease of intensity of nitrogen mineralization. Bigger decrease was stated for the humus soils. During the first 7 days average daily concentration increase of nitrate nitrogen in mineral form for the humus soils was 3,25 mg/kg/per day or 168,2 % in relation to initial state; for the the poor- humus soils it was 2,36 mg/kg/per day or 168,9 % in relation to its initial state. According to results of all 30 samples there has been statistic analisys between concetration of nitrate nitrogen int he soil after 28 days of incubation anda mount of content of total nitrogen int he soil, content of mineralized nitrogen in the soil and intensity of soil-breathing.