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undergraduate thesis
Utjecaj metode izolacije na kvalitetu DNA kod vinove loze
2024. urn:nbn:hr:151:583454
Dobiš, Lovro
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

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Dobiš, L. (2024). Utjecaj metode izolacije na kvalitetu DNA kod vinove loze (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:583454

Dobiš, Lovro. "Utjecaj metode izolacije na kvalitetu DNA kod vinove loze." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2024. https://urn.nsk.hr/urn:nbn:hr:151:583454

Dobiš, Lovro. "Utjecaj metode izolacije na kvalitetu DNA kod vinove loze." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2024. https://urn.nsk.hr/urn:nbn:hr:151:583454

Dobiš, L. (2024). 'Utjecaj metode izolacije na kvalitetu DNA kod vinove loze', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 16 December 2024, https://urn.nsk.hr/urn:nbn:hr:151:583454

Dobiš L. Utjecaj metode izolacije na kvalitetu DNA kod vinove loze [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2024 [cited 2024 December 16] Available at: https://urn.nsk.hr/urn:nbn:hr:151:583454

L. Dobiš, "Utjecaj metode izolacije na kvalitetu DNA kod vinove loze", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:151:583454

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Metadata
TitleUtjecaj metode izolacije na kvalitetu DNA kod vinove loze
Title (english)Influence of the extraction method on DNAquality in grapevine
AuthorLovro Dobiš
MentorSunčica Kujundžić (mentor)
Committee memberSonja Petrović (predsjednik povjerenstva)
Committee memberMato Drenjančević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Plant Production and Biotechnology)
Osijek
Defense date and country2024-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Genetics and Breeding of Plants, Animals and Microorganisms
Abstract
Izolacija DNA predstavlja ključni korak u provedbi molekularnih i genetskih analiza. Cilj ovoga istraživanja bio je ispitati utjecaj različitih metoda izolacije na kvalitetu DNA kod vinove loze. Istraživanje je provedeno na pet različitih sorti vinove loze (Graševina, Merlot, Frankovka, Chardonnay, Sauvignon bijeli). Genomska DNA izolirana je iz mladih listova vinove loze. Za izolaciju genomske DNA iz tkiva lista vinove loze korištene su četiri različite metode izolacije: metoda A (Nucleo Spin Plant II, PL1), metoda B (Nucleo Spin Plant II, PL2), metoda C (CTAB + 2-merkaptoetanol) i metoda D (CTAB + PVP-40). Koncentracija i čistoća izolirane DNA provjerene su korištenjem spektrofotometra i horizontalne elektroforeze. Najmanja prosječna koncentracija DNA uzoraka zabilježena je kod metode A, a najveća prosječna koncentracija DNA zabilježena je kod metode C. Svi uzorci imali su dobar omjer apsorbanci A260/A280. Svi uzorci osim onih iz metode D imali su dobar omjer apsorbanci A260/A230. Elektroforezom je potvrđena zadovoljavajuća kvaliteta uzoraka.
Abstract (english)
DNA extraction represents a key step in the implementation of molecular and genetic analyses. The aim of this research was to examine the influence of different extraction methods on DNA quality in grapevines. The research was conducted on five different grape varieties (Graševina, Merlot, Frankovka, Chardonnay, Sauvignon blanc). Genomic DNA was extracted from young vine leaves. Four different extraction methods were used to extract genomic DNA from grapevine leaf tissue: method A (Nucleo Spin Plant II, PL1), method B (Nucleo Spin Plant II, PL2), method C (CTAB + 2-mercaptoethanol) and method D (CTAB + PVP-40). The concentration and purity of the extracted DNA was checked using a spectrophotometer and horizontal electrophoresis. The lowest average concentration of DNA samples was recorded with method A, and the highest average concentration of DNA was recorded with method C. All samples had a good A260/A280 absorbance ratio. All samples except those from method D had a good A260/A230 absorbance ratio. The satisfactory quality of the samples was confirmed by electrophoresis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:583454
Study programmeTitle: BACHELOR OF PROFESSIONAL GRADUATE (BACCALAUREUS) ENGINEER OF VITICULTURE-WINEMAKING-FRUIT GROWING
Study programme type: professional
Study level: undergraduate
Academic / professional title: stručni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka vinogradarstva- vinarstva- voćarstva (stručni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka vinogradarstva- vinarstva- voćarstva)
Type of resourceText
File originBorn digital
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Created on2024-12-12 12:38:00