During three-year research period (2013-2015) carried out on the experiment fields of the Agricultural Institute of Osijek the effects of irrigation, nitrogen fertilization and genotype on the yield and quality of soybean (Glycine max (L.) Merr.) were investigated. The experiment was set up by split- split--plot design in three repetitions with the irrigation treatment as a main factor (A1 – control, A2 – rational irrigation, A3 – abundant irrigation), nitrogen fertilization was the sub-factor (B1 – control, without nitrogen fertilization; B2 – fertilization with 100 kg N ha-1 and B3 – fertilization with 200 kg N ha-1) and the second sub-factor was the soybean variety (Lucija, Vita, Ika and Tena). Analysis of variance confirmed the significant influence of irrigation of the yield, mass of 1000 grains and soybean seed production output in all research years, as well as on the content of protein and oil in 2015 and 2013, respectively. Nitrogen fertilization had influence on the mass of 1000 grains, hectoliter mass, content of protein and oil in 2013, and on germination energy in 2015, as well as on the yield in all research years. The influence of genotype had significant effect on the yield, content of water in soybean grain, mass of 1000 grains and randman of soybean seed in all research years. The genotype has significantly influenced the protein content in 2013 and 2014 and the oil content in 2014 and 2015. Germination energy and germination varied under the influence of genotype in 2014 and 2015. During the research numerous correlations determined between physical and chemical properties of grain and yield. In 2013, the yield was in poor correlation with the hectoliter mass. Strong correlations of negative direction were determined between the oil content and yield of soybean, hectoliter mass, mass of 1000 grains and protein content. In 2014, strong correlations of positive direction were determined between the mass of 1000 grains and the chemical properties of soybean grain, as well as weak correlation of negative direction between the hectoliter mass and the oil content. In 2015, the soybean yield was in positive correlation with the mass of 1000 grains and the protein content, and in negative correlation with the oil content. In 2015, chemical properties of grain had a very strong correlation of negative direction.